diff --git a/src/python/pants/backend/python/dependency_inference/parse_python_dependencies.py b/src/python/pants/backend/python/dependency_inference/parse_python_dependencies.py
index 73bfee283fb..d1624babc74 100644
--- a/src/python/pants/backend/python/dependency_inference/parse_python_dependencies.py
+++ b/src/python/pants/backend/python/dependency_inference/parse_python_dependencies.py
@@ -8,7 +8,6 @@
 from dataclasses import dataclass
 
 from pants.backend.python.dependency_inference.subsystem import PythonInferSubsystem
-from pants.backend.python.util_rules.interpreter_constraints import InterpreterConstraints
 from pants.core.util_rules.source_files import SourceFiles
 from pants.core.util_rules.stripped_source_files import strip_source_roots
 from pants.engine.collection import DeduplicatedCollection
@@ -62,7 +61,6 @@ class PythonFilesDependencies:
 @dataclass(frozen=True)
 class ParsePythonDependenciesRequest:
     source: SourceFiles
-    interpreter_constraints: InterpreterConstraints
 
 
 @dataclass(frozen=True)
@@ -113,9 +111,6 @@ async def parse_python_dependencies(
     python_infer_subsystem: PythonInferSubsystem,
 ) -> PythonFilesDependencies:
     stripped_sources = await strip_source_roots(request.source)
-    # We operate on PythonSourceField, which should be one file.
-    assert len(stripped_sources.snapshot.files) == 1
-
     native_results = await parse_python_deps(
         NativeDependenciesRequest(stripped_sources.snapshot.digest)
     )
diff --git a/src/python/pants/backend/python/dependency_inference/parse_python_dependencies_test.py b/src/python/pants/backend/python/dependency_inference/parse_python_dependencies_test.py
index d6d25189287..7de9f26533f 100644
--- a/src/python/pants/backend/python/dependency_inference/parse_python_dependencies_test.py
+++ b/src/python/pants/backend/python/dependency_inference/parse_python_dependencies_test.py
@@ -17,7 +17,6 @@
 )
 from pants.backend.python.target_types import PythonSourceField, PythonSourceTarget
 from pants.backend.python.util_rules import pex
-from pants.backend.python.util_rules.interpreter_constraints import InterpreterConstraints
 from pants.core.util_rules import stripped_source_files
 from pants.core.util_rules.source_files import SourceFiles, SourceFilesRequest
 from pants.engine.addresses import Address
@@ -49,7 +48,6 @@ def assert_deps_parsed(
     expected_imports: dict[str, ImpInfo] | None = None,
     expected_assets: list[str] | None = None,
     filename: str = "project/foo.py",
-    constraints: str = ">=3.6",
     string_imports: bool = True,
     string_imports_min_dots: int = 2,
     assets: bool = True,
@@ -82,7 +80,6 @@ def assert_deps_parsed(
                 [
                     ParsePythonDependenciesRequest(
                         source_files,
-                        InterpreterConstraints([constraints]),
                     )
                 ],
             ).path_to_deps.values()
@@ -463,7 +460,6 @@ def test_works_with_python38(rule_runner: RuleRunner) -> None:
     assert_deps_parsed(
         rule_runner,
         content,
-        constraints=">=3.8",
         expected_imports={
             "demo": ImpInfo(lineno=5, weak=False),
             "project.demo.Demo": ImpInfo(lineno=6, weak=False),
@@ -496,7 +492,6 @@ def test_works_with_python39(rule_runner: RuleRunner) -> None:
     assert_deps_parsed(
         rule_runner,
         content,
-        constraints=">=3.9",
         expected_imports={
             "demo": ImpInfo(lineno=7, weak=False),
             "project.demo.Demo": ImpInfo(lineno=8, weak=False),
diff --git a/src/python/pants/backend/python/dependency_inference/rules.py b/src/python/pants/backend/python/dependency_inference/rules.py
index 8668361c739..18606945ad2 100644
--- a/src/python/pants/backend/python/dependency_inference/rules.py
+++ b/src/python/pants/backend/python/dependency_inference/rules.py
@@ -44,7 +44,6 @@
 )
 from pants.backend.python.util_rules import ancestor_files, pex
 from pants.backend.python.util_rules.ancestor_files import AncestorFilesRequest, find_ancestor_files
-from pants.backend.python.util_rules.interpreter_constraints import InterpreterConstraints
 from pants.base.glob_match_error_behavior import GlobMatchErrorBehavior
 from pants.core import target_types
 from pants.core.target_types import AllAssetTargetsByPath, map_assets_by_path
@@ -370,14 +369,10 @@ async def _exec_parse_deps(
     field_set: PythonImportDependenciesInferenceFieldSet,
     python_setup: PythonSetup,
 ) -> PythonFileDependencies:
-    interpreter_constraints = InterpreterConstraints.create_from_field_sets(
-        [field_set], python_setup
-    )
     source = await determine_source_files(SourceFilesRequest([field_set.source]))
     resp = await parse_python_dependencies_get(
         ParsePythonDependenciesRequest(
             source,
-            interpreter_constraints,
         ),
         **implicitly(),
     )