Implements the Automatic Neuron Tracking System for Unconstrained Nematodes. This notebook takes as input raw video data from NIR and confocal microscopes and outputs neural traces and behavioral parameters for that worm.
Version 1.3.5 is the version from this article.
Version 2.1.0 is the version from this article.
Most neural network weights should be available in our Zenodo repository. We've since updated the 3D U-Net segmentation network to include NeuroPAL training data; the updated weights are available here.
Our elastix parameters are available in our registration package.
To cite this work, please refer to the following papers:
Adam A. Atanas*, Jungsoo Kim*, Ziyu Wang, Eric Bueno, McCoy Becker, Di Kang, Jungyeon Park, Talya S. Kramer, Flossie K. Wan, Saba Baskoylu, Ugur Dag, Elpiniki Kalogeropoulou, Matthew A. Gomes, Cassi Estrem, Netta Cohen, Vikash K. Mansinghka, Steven W. Flavell
Cell 2023; doi: https://doi.org/10.1016/j.cell.2023.07.035
* Equal Contribution
Adam A. Atanas, Alicia Kun-Yang Lu, Jungsoo Kim, Saba Baskoylu, Di Kang, Talya S. Kramer, Eric Bueno, Flossie K. Wan, Steven W. Flavell
bioRxiv 2024; doi: https://doi.org/10.1101/2024.07.18.601886